rsiW [2019-06-17 12:41:37]

anti-SigW

Locus: BSU01740

Isoelectric point: 6.65

Molecular weight: 23.19 kDa

Protein length: 208 aa

Gene length: 627 bp

Function: control of SigW activity

Product: anti-SigW

Essential: no

Synonyms: ybbM

Genomic Context

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Categories containing this gene/protein

• Information processing → Regulation of gene expression → Sigma factors and their control → Control of sigma factors
• Lifestyles → Coping with stress → Cell envelope stress proteins (controlled by SigM, V, W, X, Y)
• Groups of genes → Membrane proteins

Gene

Coordinates: 195,426 196,052

The protein

Protein family

• anti-sigma-W factor family (according to Swiss-Prot)

Effectors of protein activity

• degraded by RasP and PrsW under conditions of alkali shock or in the presence of antimicrobial peptides, respectively. This results in the release of SigW

Structure

• 5WUQ (the cytoplasmic domain in complex with SigW) PubMed

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: sigW-rsiW
  Description: PubMed

  Sigma factors

  • SigW: sigma factor, PubMed, in SigW regulon

  Regulatory mechanism

  • AbrB: repression, PubMed, in AbrB regulon

  Regulation

  • induced under conditions of alkali shock or in the presence of the toxin SdpC (SigW) PubMed

  Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

Mutant

• MGNA-B953 (ybbM::erm), available at the NBRP B. subtilis, Japan
• BKE01740 (rsiW::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AGGACAGCTCATTTCATCAC, downstream forward: _UP4_TAAAGCGCGTTAGCCGCTTT
• BKK01740 (rsiW::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AGGACAGCTCATTTCATCAC, downstream forward: _UP4_TAAAGCGCGTTAGCCGCTTT

Labs working on this gene/protein

• Thomas Wiegert, University of Bayreuth, Germany Homepage

References

Reviews

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Original Publications

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